GeneTex
United States (US)

PKM antibody

KO/KD
GTX111604_40492_20160616_WB_shRNA_watermark_18121410_447.jpg
GTX111604_40492_IHC_R_18121410_758.jpg
GTX111604_40492_WB_18121410_444.jpg
GTX111604_40492_20170705_IFA_18121410_523.jpg
GTX111604_40492_20161110_WB_M_R_18121410_590.jpg

Cat No. GTX111604

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTHBP antibody, MGC3932 antibody, OIP3 antibody, PK3 antibody, PKM antibody, TCB antibody, THBP1 antibody, PKM2 antibody, PK, muscle type antibody, tumor M2-PK antibody, OIP-3 antibody, pyruvate kinase 2/3 antibody, pyruvate kinase muscle isozyme antibody, OPA-interacting protein 3 antibody, p58 antibody, pyruvate kinase isozymes M1/M2 antibody, thyroid hormone-binding protein 1 antibody, cytosolic thyroid hormone-binding protein antibody, thyroid hormone-binding protein, cytosolic antibody, pyruvate kinase, muscle antibody

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX111604_40492_20160616_WB_shRNA_watermark_18121410_447.jpg

GTX111604 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:10000.

GTX111604_40492_IHC_R_18121410_758.jpg

GTX111604 IHC-P Image

Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Pyruvate Kinase (muscle) antibody (GTX111604) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111604_40492_WB_18121410_444.jpg

GTX111604 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX111604 diluted at 1:5000

GTX111604_40492_20170705_IFA_18121410_523.jpg

GTX111604 ICC/IF Image

PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM protein stained by PKM antibody (GTX111604) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX111604_40492_20161110_WB_M_R_18121410_590.jpg

GTX111604 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:5000.

SDS
Glycerol.pdf
Thimerosal.pdf