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PKM antibody

Anti-PKM antibody used in Western Blot (WB). GTX111604
Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX111604
Anti-PKM antibody used in Western Blot (WB). GTX111604
Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111604
Anti-PKM antibody used in Western Blot (WB). GTX111604

Cat No. GTX111604

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Kinase M1/2,Cthbp,Hel-S-30,Oip3,Pk3,Pkm2,Tcb,Thbp1,Pkm

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

DATA IMAGES
Anti-PKM antibody used in Western Blot (WB). GTX111604

GTX111604 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:10000.

Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX111604

GTX111604 IHC-P Image

Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Pyruvate Kinase (muscle) antibody (GTX111604) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKM antibody used in Western Blot (WB). GTX111604

GTX111604 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX111604 diluted at 1:5000

Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111604

GTX111604 ICC/IF Image

PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM protein stained by PKM antibody (GTX111604) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PKM antibody used in Western Blot (WB). GTX111604

GTX111604 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:5000.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169