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PKM1 antibody

Anti-PKM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50734
Anti-PKM1 antibody used in Western Blot (WB). GTX50734

Cat. No. GTX50734

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PKM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Product Note

This antibody was raised against a synthetic peptide corresponding to a region within aa 399-403 of human PKM1 (UniProt #P14618-2). Based on a BLAST result, it is specific for PKM1 and does not cross with PKM2.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 399~403(V-R-A-S-S) derived from human PKM1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174754

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase M1/2 , CTHBP , HEL-S-30 , OIP3 , PK3 , PKM2 , TCB , THBP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-PKM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50734

GTX50734 ICC/IF Image

ICC/IF analysis of methanol-fixed MEF cells using GTX50734 PKM1 antibody.

Anti-PKM1 antibody used in Western Blot (WB). GTX50734

GTX50734 WB Image

WB analysis of extracts from U87 cells using GTX50734 PKM1 antibody.

REFERENCE

Application Reference

Li C et al. Cancer Chemother Pharmacol 2016; 78 (6) : 1199-1207 linc-ror confers * resistance to pancreatic cancer cells via inducing autophagy and modulating the mir-124/ptbp1/pkm2 axis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399