GeneTex
United States (US)

PKNOX1 antibody

GTX114991_40870_IP_18121410_922.jpg
GTX114991_40870_WB_18121410_386.jpg
GTX114991_40870_IFA_18121410_693.jpg

Cat No. GTX114991

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ChIP-seq
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:1000
ChIP-seq Assay dependent

Note :

IP
target validated by Mass Spectrometry

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HepG2 nucleus

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKNOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pbx/Knotted 1 Homeobox 1,Prep1,Pkonx1C,Pknox1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
GTX114991_40870_IP_18121410_922.jpg

GTX114991 IP Image

PKNOX1 antibody immunoprecipitates PKNOX1 ?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 ?protein by 2 μg of PKNOX1 ?antibody (GTX114991) 10% SDS-PAGE The immunoprecipitated PKNOX1 ?protein was detected by PKNOX1 ?antibody (GTX114991) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114991_40870_WB_18121410_386.jpg

GTX114991 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HepG2 nucleus
10% SDS PAGE
GTX114991 diluted at 1:2000

GTX114991_40870_IFA_18121410_693.jpg

GTX114991 ICC/IF Image

PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKNOX1 protein stained by PKNOX1 antibody (GTX114991) diluted at 1:1000.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf