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PKR antibody [GT1150]

Recombinant antibody
Anti-PKR antibody [GT1150] used in Western Blot (WB). GTX00929
Anti-PKR antibody [GT1150] used in Western Blot (WB). GTX00929

Cat. No. GTX00929

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1150

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all PKR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan PKR

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 2 , EIF2AK1 , PKR , PPP1R83 , PRKR

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PKR antibody [GT1150] used in Western Blot (WB). GTX00929

GTX00929 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKR antibody [GT1150] (GTX00929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30680794

Anti-PKR antibody [GT1150] used in Western Blot (WB). GTX00929

GTX00929 WB Image

WB analysis of various samples using GTX00929 PKR antibody [GT1150].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399