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PKR antibody

Anti-PKR antibody used in Western Blot (WB). GTX31746
Anti-PKR antibody used in Western Blot (WB). GTX31746
Anti-PKR antibody used in IHC (Paraffin sections) (IHC-P). GTX31746
Anti-PKR antibody used in Western Blot (WB). GTX31746

Cat. No. GTX31746

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Rat
Package
100 μg ($399)
See all PKR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Rat Lung Tissue Lysate(GTX27941) , MCF7 Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PKR antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PKR.The immunogen is located within the first 50 amino acids of PKR.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 2 , EIF2AK1 , PKR , PPP1R83 , PRKR

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PKR antibody used in Western Blot (WB). GTX31746

GTX31746 WB Image

WB analysis of MCF7 cell lysate using GTX31746 PKR antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PKR antibody used in Western Blot (WB). GTX31746

GTX31746 WB Image

WB analysis of various sample lysates using GTX31746 PKR antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-PKR antibody used in IHC (Paraffin sections) (IHC-P). GTX31746

GTX31746 IHC-P Image

IHC-P analysis of rat lung tissue using GTX31746 PKR antibody.
Working concentration : 5 μg/ml

Anti-PKR antibody used in Western Blot (WB). GTX31746

GTX31746 WB Image

WB analysis of various sample lysates using GTX31746 PKR antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399