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PKR antibody

Orthogonal
Anti-PKR antibody used in Western Blot (WB). GTX50591
Anti-PKR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50591
Anti-PKR antibody used in IHC (Paraffin sections) (IHC-P). GTX50591

Cat. No. GTX50591

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all PKR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.444~448 (K-R-T-R-S) derived from human PKR.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 2 , EIF2AK1 , PKR , PPP1R83 , PRKR

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PKR antibody used in Western Blot (WB). GTX50591

GTX50591 WB Image

WB analysis of extracts from 293, HeLa, and C6 cells using GTX50591 PKR antibody.

Anti-PKR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50591

GTX50591 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50591 PKR antibody.

Anti-PKR antibody used in IHC (Paraffin sections) (IHC-P). GTX50591

GTX50591 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50591 PKR antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399