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PKR antibody

Anti-PKR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53983
Anti-PKR antibody used in Western Blot (WB). GTX53983
Anti-PKR antibody used in IHC (Paraffin sections) (IHC-P). GTX53983

Cat No. GTX53983

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:100-1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 2 , EIF2AK1 , PKR , PPP1R83 , PRKR

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PKR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53983

GTX53983 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX53983 PKR antibody.
Blue : DAPI
Dilution : 1:100

Anti-PKR antibody used in Western Blot (WB). GTX53983

GTX53983 WB Image

WB analysis of various sample lysates using GTX53983 PKR antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-PKR antibody used in IHC (Paraffin sections) (IHC-P). GTX53983

GTX53983 IHC-P Image

IHC-P analysis of human liver tissue using GTX53983 PKR antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf