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PKR (phospho Thr446) antibody

Anti-PKR (phospho Thr446) antibody used in Western Blot (WB). GTX132789

Cat No. GTX132789

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

IFN-r treated HeLa

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human PKR (phospho Thr446). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 2,EIF2AK1,PKR,PPP1R83,PRKR

Background

EIF2AK2 is a protein serine/threonine kinase that acquires enzymatic activity following autophosphorylation, a process mediated by double-stranded RNA (dsRNA). Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha; MIM 603907), leading to the inhibition of protein synthesis (summary by Kuhen et al., 1996 [PubMed 8812437]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PKR (phospho Thr446) antibody used in Western Blot (WB). GTX132789

GTX132789 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKR (phospho Thr446) antibody (GTX132789) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf