GeneTex
United States (US)

PKR (phospho Thr451) antibody

GTX32348_20190131_WB_19013102_774.jpg
GTX32348_20190131_IHC-P_19013102_326.jpg

Cat No. GTX32348

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PKR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2,Eif2Ak1,Pkr,Ppp1R83,Prkr,Eif2Ak2

Cellular Localization

Cytoplasm,Nucleus,Perinuclear region

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES
GTX32348_20190131_WB_19013102_774.jpg

GTX32348 WB Image

WB analysis of PMA-treated HeLa (A), H2O2-treated Raw264.7 (B), LPS-treated NIH3T3 (C) whole cell lysates using GTX32348 PKR (phospho Thr451) antibody.

GTX32348_20190131_IHC-P_19013102_326.jpg

GTX32348 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32348 PKR (phospho Thr451) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Package List Price ($)
$ 319