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PLA2G1B antibody, C-term

Anti-PLA2G1B antibody, C-term used in Western Blot (WB). GTX88948
Anti-PLA2G1B antibody, C-term used in Western Blot (WB). GTX88948

Cat. No. GTX88948

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all PLA2G1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01μg/ml
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88948-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence NKAHKNLDTKKYCQS, from the C Terminus of the protein sequence according to NP_000919.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 group IB , PLA2 , PLA2A , PPLA2

Cellular Localization

Secreted

Background

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-PLA2G1B antibody, C-term used in Western Blot (WB). GTX88948

GTX88948 WB Image

WB analysis of HEK293 overexpressing PLA2G1B (mock transfection in first lane) using GTX88948 PLA2G1B antibody, C-term.

Anti-PLA2G1B antibody, C-term used in Western Blot (WB). GTX88948

GTX88948 WB Image

WB analysis of human pancreas lysate using GTX88948 PLA2G1B antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399