GeneTex
United States (US)

PLA2G4A antibody, Internal

GTX88072_2540_WB_18121411_688.jpg

Cat No. GTX88072

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88072-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholipase A2 Group Iva,Pla2G4,Cpla2,Cpla2-Alpha,Pla2G4A

Cellular Localization

Translocates to membrane vesicles in a calcium-dependent fashion.

Background

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88072_2540_WB_18121411_688.jpg

GTX88072 WB Image

WB analysis of HeLa lysate using GTX88072 PLA2G4A antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329