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PLA2G4A antibody

Anti-PLA2G4A antibody used in Western Blot (WB). GTX110218
Anti-PLA2G4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110218
Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX110218
Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX110218
Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX110218
Anti-PLA2G4A antibody used in Western Blot (WB). GTX110218

Cat. No. GTX110218

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PLA2G4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PLA2G4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951272

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 group IVA , PLA2G4 , cPLA2 , cPLA2-alpha

Cellular Localization

Cytoplasm , Cytoplasmic vesicle

Background

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PLA2G4A antibody used in Western Blot (WB). GTX110218

GTX110218 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLA2G4A antibody (GTX110218) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PLA2G4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110218

GTX110218 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using cPLA 2 (GTX110218) antibody at 1:200 dilution.

Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX110218

GTX110218 IHC-P Image

PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PLA2G4A antibody (GTX110218) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX110218

GTX110218 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PLA2G4A(GTX110218) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX110218

GTX110218 IHC-P Image

PLA2G4A antibody detects PLA2G4A protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PLA2G4A antibody (GTX110218) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLA2G4A antibody used in Western Blot (WB). GTX110218

GTX110218 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX110218 diluted at 1:1000

REFERENCE

Application Reference

Xu S et al. Transl Oncol 2023; 39 : 101817 Thrombin induces ferroptosis in triple-negative breast cancer through the cPLA2α/ACSL4 signaling pathway.
Ueno H et al. Am J Respir Cell Mol Biol 2020; Cysteinyl Leukotriene synthesis via Phospholipase A2 Group IV Mediates Exercise-induced Bronchoconstriction and Airway Remodeling.
Application : WB
Reactivity : Mouse
Guo P et al. Cancer Biol Med 2019; 16 (2) : 377-390 Cytosolic phospholipase A2α modulates cell-matrix adhesion via the FAK/paxillin pathway in hepatocellular carcinoma.
Application : WB
Reactivity : Human
Chen L et al. Cell Death Dis 2017; 8 (4) : e2728 cPLA2α mediates TGF-β-induced epithelial-mesenchymal transition in breast cancer through PI3k/Akt signaling.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169