GeneTex

  • Country / Location Selection

United States (US)

PLA2G4A antibody

Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX117583
Anti-PLA2G4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117583
Anti-PLA2G4A antibody used in Western Blot (WB). GTX117583

Cat. No. GTX117583

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PLA2G4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLA2G4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163026

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 group IVA , PLA2G4 , cPLA2 , cPLA2-alpha

Cellular Localization

Cytoplasm , Cytoplasmic vesicle

Background

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PLA2G4A antibody used in IHC (Paraffin sections) (IHC-P). GTX117583

GTX117583 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using PLA2G4A(GTX117583) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PLA2G4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117583

GTX117583 ICC/IF Image

PLA2G4A antibody detects PLA2G4A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PLA2G4A protein stained by PLA2G4A antibody (GTX117583) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PLA2G4A antibody used in Western Blot (WB). GTX117583

GTX117583 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX117583 diluted at 1:3000

REFERENCE

Application Reference

Yuan C et al. J Biol Chem 2015; 290 (9) : 5606-20 A Cyclooxygenase-2-dependent Prostaglandin E2 Biosynthetic System in the Golgi Apparatus.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PLA2G4A antibody (GTX117583). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169