GeneTex
United States (US)

PLA2G4A (phospho Ser505) antibody

GTX32342_20190131_WB_19013101_645.jpg
GTX32342_20190131_IHC-P_19013101_733.jpg
GTX32342_20190131_ICCIF_19013101_516.jpg

Cat No. GTX32342

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

85 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of PLA2G4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholipase A2 Group Iva,Pla2G4,Cpla2,Cpla2-Alpha,Pla2G4A

Cellular Localization

Cytoplasm,Cytoplasmic vesicle

Background

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES
GTX32342_20190131_WB_19013101_645.jpg

GTX32342 WB Image

WB analysis of lambda protein phosphatase-treated HeLa (A), lambda protein phosphatase-treated NIH3T3 (B) whole cell lysates using GTX32342 PLA2G4A (phospho Ser505) antibody.

GTX32342_20190131_IHC-P_19013101_733.jpg

GTX32342 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32342 PLA2G4A (phospho Ser505) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32342_20190131_ICCIF_19013101_516.jpg

GTX32342 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32342 PLA2G4A (phospho Ser505) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319