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PLA2G4F antibody [N3C2], Internal

Anti-PLA2G4F antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120598
Anti-PLA2G4F antibody [N3C2], Internal used in Western Blot (WB). GTX120598

Cat. No. GTX120598

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PLA2G4F products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLA2G4F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 group IVF , PLA2G4FZ

Background

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.

Database

Research Area

DATA IMAGES

Anti-PLA2G4F antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120598

GTX120598 ICC/IF Image

PLA2G4F antibody [N3C2], Internal detects PLA2G4F protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLA2G4F protein stained by PLA2G4F antibody [N3C2], Internal (GTX120598) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PLA2G4F antibody [N3C2], Internal used in Western Blot (WB). GTX120598

GTX120598 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX120598 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169