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PLA2G5 antibody [3G1]

Anti-PLA2G5 antibody [3G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79469

Cat. No. GTX79469

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G1

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PLA2G5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 2 μg/ml
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length W79A human group V phospholipase A2.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11165513

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 group V , FRFB , GV-PLA2 , PLA2-10 , hVPLA(2)

Cellular Localization

Secreted

Background

This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PLA2G5 antibody [3G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79469

GTX79469 ICC/IF Image

ICC/IF analysis of NCCIT cells using GTX79469 PLA2G5 antibody [3G1]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399