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PLA2G6 antibody [N3C3]

Anti-PLA2G6 antibody [N3C3] used in Western Blot (WB). GTX103717
Anti-PLA2G6 antibody [N3C3] used in Western Blot (WB). GTX103717

Cat. No. GTX103717

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all PLA2G6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLA2G6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169779

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 group VI , CaI-PLA2 , GVI , INAD1 , IPLA2-VIA , NBIA2 , NBIA2A , NBIA2B , PARK14 , PLA2 , PNPLA9 , iPLA2 , iPLA2beta

Cellular Localization

Isoform LH-iPLA2: Membrane; Peripheral membrane protein , Isoform SH-iPLA2: Cytoplasm

Background

The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PLA2G6 antibody [N3C3] used in Western Blot (WB). GTX103717

GTX103717 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX103717 diluted at 1:500

Anti-PLA2G6 antibody [N3C3] used in Western Blot (WB). GTX103717

GTX103717 WB Image

Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PLA2G6 antibody [N3C3] (GTX103717) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169