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PLA2R1 antibody, Internal

Anti-PLA2R1 antibody, Internal used in Western Blot (WB). GTX88257

Cat. No. GTX88257

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Pig
Package
100 μg ($399)
See all PLA2R1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

169 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88257-PEP).

Product Note

This antibody is expected to recognize all reported isoforms (NP_031392.3; NP_001007268.1; NP_001182570.1).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EKKKDTPKQHGT, from the Internal region of the protein sequence according to NP_031392.3; NP_001007268.1; NP_001182570.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724788

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase A2 receptor 1 , CLEC13C , PLA2-R , PLA2G1R , PLA2IR , PLA2R

Cellular Localization

Cell membrane

Background

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-PLA2R1 antibody, Internal used in Western Blot (WB). GTX88257

GTX88257 WB Image

GTX88257 (1μg/ml) staining of Human Ileum lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

REFERENCE

Application Reference

Takahashi S et al. FEBS Lett 2015; 589 (7) : 829-35 C-type lectin-like domain and fibronectin-like type II domain of phospholipase A(2) receptor 1 modulate binding and migratory responses to collagen.
Application : ELISA
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399