GeneTex
United States (US)

PLAP antibody [N3C2], Internal

GTX115226_40835_IP_18121410_722.jpg
GTX115226_40835_WB_18121410_301.jpg

Cat No. GTX115226

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

NT2D1 , U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholipase A2 Activating Protein,Doa1,Ndmsba,Pla2P,Plap,Plaa

Background

Plays an important role in the regulation of specific inflammatory disease processes.

Database

DATA IMAGES
GTX115226_40835_IP_18121410_722.jpg

GTX115226 IP Image

PLAP antibody [N3C2], Internal immunoprecipitates PLAP protein in IP experiments. IP Sample: NT2D1 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of PLAP by 3 μg of PLAP antibody [N3C2], Internal (GTX115226) 7.5% SDS-PAGE The immunoprecipitated PLAP protein was detected by PLAP antibody [N3C2], Internal (GTX115226) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX115226_40835_WB_18121410_301.jpg

GTX115226 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: U87-MG
7.5% SDS PAGE
GTX115226 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169