APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human PLCE1, corresponding to a region within C-terminal amino acids.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phospholipase C epsilon 1 , NPHS3 , PLCE , PPLC
Cellular Localization
Cytoplasm, cytosol , Cell membrane , Golgi apparatus membrane
Background
This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
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GTX02580 IHC-P Image
IHC-P analysis of human normal tissues adjacent to pancreatic cancer using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
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GTX02580 IHC-P Image
IHC-P analysis of human pancreatic cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
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GTX02580 IHC-P Image
IHC-P analysis of human colorectal cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
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GTX02580 WB Image
WB analysis of VERO cell lysate using GTX02580 PLCE1 Antibody. The lane on the left was treated with blocking peptide.
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GTX02580 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
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GTX02580 IHC-P Image
IHC-P analysis of mouse colon tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
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GTX02580 IHC-P Image
IHC-P analysis of human normal tissues adjacent to colorectal cancer using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
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GTX02580 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX02580 PLCE1 antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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GTX02580 IHC-P Image
IHC-P analysis of rat thymus tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
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GTX02580 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
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|
GTX02580 WB Image
WB analysis of HEK293 cell lysate using GTX02580 PLCE1 Antibody.
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GTX02580 IHC-P Image
IHC-P analysis of human pancreatic cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
|
|
GTX02580 IHC-P Image
IHC-P analysis of human colorectal cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
|
|
GTX02580 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
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GTX02580 IHC-P Image
IHC-P analysis of rat colon tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
|
|
|
GTX02580 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100
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REFERENCE
There are currently no references for PLCE1 antibody (GTX02580). Be the first to share your publications with this product.
REVIEW
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