GeneTex
United States (US)

PLCG1 antibody

Cat No. GTX30787

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

For IP: Use at a dilution of 1:50. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

149 kDa. ( Note )

Specificity/Sensitivity

Recognizes endogenous levels of total human PLC gamma 1 protein. Does not crossreact with PLC gamma 2
PROPERTIES

Form

Liquid

Buffer

10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA containing 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the C-terminal residues of human PLCG1.

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholipase C Gamma 1,Nckap3,Plc-Ii,Plc1,Plc148,Plcgamma1,Plcg1

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

Package List Price ($)
$ 319