For IP: Use at a dilution of 1:50. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the end user.
Recognizes endogenous levels of total human PLC gamma 1 protein. Does not crossreact with PLC gamma 2
10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA containing 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.05 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide corresponding to the C-terminal residues of human PLCG1.
Protein A affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Phospholipase C Gamma 1,Nckap3,Plc-Ii,Plc1,Plc148,Plcgamma1,Plcg1
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.