GeneTex

  • Country / Location Selection

United States (US)

PLCG1 (phospho Tyr1253) antibody

Anti-PLCG1 (phospho Tyr1253) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32238
Anti-PLCG1 (phospho Tyr1253) antibody used in Western Blot (WB). GTX32238

Cat. No. GTX32238

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PLCG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

149 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PLCG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887644

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C gamma 1 , NCKAP3 , PLC-II , PLC1 , PLC148 , PLCgamma1

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PLCG1 (phospho Tyr1253) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32238

GTX32238 ICC/IF Image

ICC/IF analysis of formalin-fixed A431 cells using GTX32238 PLCG1 (phospho Tyr1253) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-PLCG1 (phospho Tyr1253) antibody used in Western Blot (WB). GTX32238

GTX32238 WB Image

WB analysis of EGF-treated MCF7 (A), A431 (B), mouse brain (C), mouse liver (D), EGF-treated H9C2 (E) whole cell lysates using GTX32238 PLCG1 (phospho Tyr1253) antibody.

REFERENCE

Application Reference

Kang DS et al. EMBO Rep 2018; (Epub) Netrin-1/DCC-mediated PLCγ1 activation is required for axon guidance and brain structure development.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for PLCG1 (phospho Tyr1253) antibody (GTX32238). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399