GeneTex
United States (US)

PLCG1 (phospho Tyr1253) antibody

GTX32238_20190131_ICCIF_19013101_790.jpg
GTX32238_20190131_WB_19013101_842.jpg

Cat No. GTX32238

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

149 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PLCG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PLCgamma1 Antibody , PLC148 Antibody , NCKAP3 Antibody , phospholipase C, gamma 1 Antibody , PLCG1 Antibody , PLC-II Antibody , PLC1 Antibody

Cellular Localization

Cell projection,Lamellipodium,Ruffle

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32238_20190131_ICCIF_19013101_790.jpg

GTX32238 ICC/IF Image

ICC/IF analysis of formalin-fixed A431 cells using GTX32238 PLCG1 (phospho Tyr1253) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32238_20190131_WB_19013101_842.jpg

GTX32238 WB Image

WB analysis of EGF-treated MCF7 (A), A431 (B), mouse brain (C), mouse liver (D), EGF-treated H9C2 (E) whole cell lysates using GTX32238 PLCG1 (phospho Tyr1253) antibody.

REFERENCE
Package List Price ($)
$ 319