*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
NIH-3T3 (starvation for 16 hr and 200 ng/ml EGF treatment for 30 min) , A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min)
Rat, Bovine, Xenopus laevis(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.27 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide corresponding to residues around human PLCG1 (phospho Tyr783). The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Phospholipase C Gamma 1,Nckap3,Plc-Ii,Plc1,Plc148,Plcgamma1,Plcg1
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX133465 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG1 (phospho Tyr783) antibody (GTX133465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133465 WB Image
Untreated (–) and treated (+) A431 whole cell extract (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLCG1 (phospho Tyr783) antibody (GTX133465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.