GeneTex
United States (US)

PLCG2 antibody [N1N2], N-term

GTX111293_WB_19011507_654.jpg
GTX111293_40065_IHC_19011507_670.jpg
GTX111293_40065_20170915_WB_M_19011507_386.jpg
GTX111293_IFA_19011507_773.jpg

Cat No. GTX111293

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Positive Control

Raji , Raw264.7

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PLCG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PLCG2 antibody, phospholipase C-IV antibody, phospholipase C gamma 2 antibody, phospholipase C, gamma 2 (phosphatidylyinositol-specific) antibody, phospholipase C-gamma-2 antibody, phosphoinositide phospholipase C-gamma-2 antibody, PLC-IV antibody, PLC-gamma-2 antibody, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 antibody, phospholipase C, gamma 2 (phosphatidylinositol-specific) antibody

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide-specific phospholipase C enzymes, C-beta is regulated by heterotrimeric G protein-coupled receptors, while the closely related C-gamma-1 (PLCG1; MIM 172420) and C-gamma-2 enzymes are controlled by receptor tyrosine kinases. The C-gamma-1 and C-gamma-2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX111293_WB_19011507_654.jpg

GTX111293 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
5% SDS PAGE
GTX111293 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111293_40065_IHC_19011507_670.jpg

GTX111293 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using PLCG2(GTX111293) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111293_40065_20170915_WB_M_19011507_386.jpg

GTX111293 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 antibody [N1N2], N-term (GTX111293) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111293_IFA_19011507_773.jpg

GTX111293 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PLC gamma 2(GTX111293) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169