GeneTex
United States (US)

PLCG2 (phospho Tyr753) antibody

GTX38559_20190129_WB_19012901_487.jpg
GTX38559_20190129_ICCIF_19012901_351.jpg

Cat No. GTX38559

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Specificity/Sensitivity

This antibody detects endogenous levels of PLC gamma 2 only when phosphorylated at tyrosine 753.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of tyrosine 753 (S -L-Y(p)-D-V).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholipase C Gamma 2,Aplaid,Fcas3,Plc-Iv,Plc-Gamma-2,Plcg2

Background

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
GTX38559_20190129_WB_19012901_487.jpg

GTX38559 WB Image

WB analysis of extracts of HepG2 cells treated or untreated with EGF using GTX38559 PLCG2 (phospho Tyr753) antibody.

GTX38559_20190129_ICCIF_19012901_351.jpg

GTX38559 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX38559 PLCG2 (phospho Tyr753) antibody.

Package List Price ($)
$ 219