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PLCG2 (phospho Tyr753) antibody

Anti-PLCG2 (phospho Tyr753) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50225
Anti-PLCG2 (phospho Tyr753) antibody used in Western Blot (WB). GTX50225

Cat. No. GTX50225

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all PLCG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from human PLCG2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C gamma 2 , APLAID , FCAS3 , PLC-IV , PLC-gamma-2

Background

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-PLCG2 (phospho Tyr753) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50225

GTX50225 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50225 PLCG2 (phospho Tyr753) antibody.

Anti-PLCG2 (phospho Tyr753) antibody used in Western Blot (WB). GTX50225

GTX50225 WB Image

WB analysis of extracts from HepG2 cells untreated or treated with EGF using GTX50225 PLCG2 (phospho Tyr753) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399