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PLCG2 (phospho Tyr753) antibody

Orthogonal
Anti-PLCG2 (phospho Tyr753) antibody used in Western Blot (WB). GTX78962

Cat. No. GTX78962

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all PLCG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PLCγ2 around the phosphorylation site of tyrosine 753 (S-L-Yp-D-V).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11179671

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APLAID,FCAS3,PLCG2,PLCIV,PLCgamma2,phospholipase C gamma 2,PLC gamma2,PLC gamma 2

Background

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-PLCG2 (phospho Tyr753) antibody used in Western Blot (WB). GTX78962

GTX78962 WB Image

Orthogonal

WB analysis of A431 cells, untreated or treated with EGF (200ng/ml, 5min) lysates using GTX78962 PLCG2 (phospho Tyr753) antibody (Lane 3 and 4) and PLCG2 antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399