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PLCG2 (phospho Tyr759) antibody

Orthogonal
Anti-PLCG2 (phospho Tyr759) antibody used in Western Blot (WB). GTX133463

Cat. No. GTX133463

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PLCG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Positive Control

Pervanadate treated Raji cell

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr759 of human PLCG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2868555

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C gamma 2 , APLAID , FCAS3 , PLC-IV , PLC-gamma-2

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide-specific phospholipase C enzymes, C-beta is regulated by heterotrimeric G protein-coupled receptors, while the closely related C-gamma-1 (PLCG1; MIM 172420) and C-gamma-2 enzymes are controlled by receptor tyrosine kinases. The C-gamma-1 and C-gamma-2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PLCG2 (phospho Tyr759) antibody used in Western Blot (WB). GTX133463

GTX133463 WB Image

Orthogonal

Untreated (–) and treated (+) Raji whole cell extracts (10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLCG2 (phospho Tyr759) antibody (GTX133463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Y Ye et al. Platelets 2024; 35 (1) : 2308635 Antiplatelet effects of the CEACAM1-derived peptide QDTT
Ye Y et al. Front Pharmacol 2023; 14 : 1255069 Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis.
Ye Y et al. Biosci Biotechnol Biochem 2020; 84 (1) : 85-94 The CEACAM1-derived peptide QLSN impairs collagen-induced human platelet activation through glycoprotein VI.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169