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PLCH1 antibody [N2C1], Internal

Anti-PLCH1 antibody [N2C1], Internal used in Western Blot (WB). GTX108612
Anti-PLCH1 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX108612

Cat. No. GTX108612

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PLCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

189 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLCH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951280

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C eta 1 , PLCL3

Cellular Localization

Cytoplasm , Membrane

Background

PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PLCH1 antibody [N2C1], Internal used in Western Blot (WB). GTX108612

GTX108612 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hep G2 (GTX27900)
5% SDS PAGE
GTX108612 diluted at 1:500

Anti-PLCH1 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX108612

GTX108612 IHC-Fr Image

The data was published in the journal Sci Rep in 2017. PMID: 28821749

REFERENCE

Application Reference

Ichrak Drissi et al. J Med Genet 2022; 59 (4) : 358-365 Mutations in phospholipase C eta-1 (PLCH1) are associated with holoprosencephaly.
Application : IHC-P
Reactivity : Human
Chomyk AM et al. Sci Rep 2017; 7 (1) : 8696 DNA methylation in demyelinated multiple sclerosis hippocampus.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169