Neutralizing/Blocking assay analysis of mouse skin endothelial cells (SENDs) were treated with or without PlGF2 (20ng/ml) in the absence or presence of 5 or 20 μg/ml GTX53460 PLGF antibody [9G33] for 30 mins. Phospho-VEGFR1 was detected with IP-Western for pTyrosine.