GeneTex
United States (US)

PLGF antibody

GTX101023_39812_IHC_19011506_316.jpg
GTX101023_39812_WB_19011506_742.jpg

Cat No. GTX101023

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLGF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Placental Growth Factor,D12S1900,Pgfl,Pigf,Plgf,Plgf-2,Shgc-10760,Pgf

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES
GTX101023_39812_IHC_19011506_316.jpg

GTX101023 IHC-P Image

PLGF antibody detects PGF protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PLGF antibody (GTX101023) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101023_39812_WB_19011506_742.jpg

GTX101023 WB Image

PLGF antibody detects PGF protein by Western blot analysis.
A. 30 µg Raji whole cell lysate/extract
B. 30 µg K562 whole cell lysate/extract
C. 30 µg THP-1 whole cell lysate/extract
15 % SDS-PAGE
PLGF antibody (GTX101023) dilution: 1:500

SDS
Glycerol.pdf
Thimerosal.pdf