GeneTex
United States (US)

PLK1 antibody [13E8]

KO/KD Orthogonal
GTX15779_1531_WB_19011519_960.jpg
GTX15779_307_ICC-IF_19011519_534.jpg
GTX15779_309_ICC-IF_19011519_403.jpg
GTX15779_306_ICC-IF_19011519_680.jpg
GTX15779_1532_WB_19011519_940.jpg
GTX15779_1039_IHC-P_19011519_322.jpg
GTX15779_310_ICC-IF_19011519_552.jpg
GTX15779_1533_WB_19011519_459.jpg
GTX15779_308_ICC-IF_19011519_531.jpg

Cat No. GTX15779

Host Mouse
Clonality Monoclonal
Clone Name 13E8
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 3 μg/ml
IHC-P 1:20
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein containing residues 300-400 of human PLK1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Polo Like Kinase 1,Plk,Stpk13,Plk1

Cellular Localization

Centromere,Centrosome,Chromosome,Cytoplasm,Cytoskeleton,Kinetochore,Microtubule organizing center,Midbody,Nucleus,Spindle

Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
GTX15779_1531_WB_19011519_960.jpg

GTX15779 WB Image

KO/KD

WB analysis of PLK1 knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15779 PLK1 antibody [13E8].
Dilution : 1 µg/ml

GTX15779_307_ICC-IF_19011519_534.jpg

GTX15779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8].
Progression of cells through subsequent phases of the cell cycle results in differential localization of PLK1 in spindle poles, kinetochores and cleavage furrow.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 3 µg/ml in 0.1% BSA and incubated for overnight at 4°C

GTX15779_309_ICC-IF_19011519_403.jpg

GTX15779 ICC/IF Image

ICC/IF analysis of WiDr cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15779_306_ICC-IF_19011519_680.jpg

GTX15779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 3 µg/ml in 0.1% BSA and incubated for overnight at 4°C

GTX15779_1532_WB_19011519_940.jpg

GTX15779 WB Image

KO/KD Orthogonal

WB analysis of untreated or Nocodazole (100nM, 48 hours) treated U2OS lysate from non-targeting control or PLK1 siRNA transfected U2OS cells using GTX15779 PLK1 antibody [13E8].
Dilution : 1:1000
Loading : 25 µg

GTX15779_1039_IHC-P_19011519_322.jpg

GTX15779 IHC-P Image

IHC-P analysis of human colon tissue using GTX15779 PLK1 antibody [13E8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15779_310_ICC-IF_19011519_552.jpg

GTX15779 ICC/IF Image

ICC/IF analysis of U251 cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15779_1533_WB_19011519_459.jpg

GTX15779 WB Image

Orthogonal

WB analysis of whole cell extracts (30µg) of HeLa (Lane 1), treated with Nocodazole (500nM for 18 hrs) (Lane2), SW-480 (Lane 3), treated with Nocodazole (500nM for 18 hrs) (Lane 4), COLO 205 (Lane 5), HCT 116 (Lane 6), K-562 (Lane 7), MDA-MB-231 (Lane 8), HepG2 (Lane 9), A549 (Lane 10) using GTX15779 PLK1 antibody [13E8].
Dilution : 1:1000

GTX15779_308_ICC-IF_19011519_531.jpg

GTX15779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

Package List Price ($)
$ 319