PLK1 antibody [13E8]

ICC/IF analysis of WiDr cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8].
Progression of cells through subsequent phases of the cell cycle results in differential localization of PLK1 in spindle poles, kinetochores and cleavage furrow.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC

IHC-P analysis of human colon tissue using GTX15779 PLK1 antibody [13E8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

WB analysis of untreated or Nocodazole (100nM, 48 hours) treated U2OS lysate from non-targeting control or PLK1 siRNA transfected U2OS cells using GTX15779 PLK1 antibody [13E8].
Dilution : 1:1000
Loading : 25 μg

ICC/IF analysis of U251 cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

WB analysis of whole cell extracts (30μg) of HeLa (Lane 1), treated with Nocodazole (500nM for 18 hrs) (Lane2), SW-480 (Lane 3), treated with Nocodazole (500nM for 18 hrs) (Lane 4), COLO 205 (Lane 5), HCT 116 (Lane 6), K-562 (Lane 7), MDA-MB-231 (Lane 8), HepG2 (Lane 9), A549 (Lane 10) using GTX15779 PLK1 antibody [13E8].
Dilution : 1:1000

WB analysis of PLK1 knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15779 PLK1 antibody [13E8].
Dilution : 1 μg/ml

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC