PLK1 antibody [13E8]

Cat. No. GTX15779

Host	Mouse
Clonality	Monoclonal
Clone Name	13E8
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity	Human, Mouse, Rat
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX15779 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	3 μg/ml
IHC-P	1:20
IP	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein containing residues 300-400 of human PLK1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 1 , PLK , STPK13

Cellular Localization

Nucleus

Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Database

Gene ID: 5347 PLK1
UniProt: P53350 PLK1

Research Area

DATA IMAGES

GTX15779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC

GTX15779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

GTX15779 WB Image

WB analysis of PLK1 knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15779 PLK1 antibody [13E8].
Dilution : 1 μg/ml

GTX15779 WB Image

WB analysis of whole cell extracts (30μg) of HeLa (Lane 1), treated with Nocodazole (500nM for 18 hrs) (Lane2), SW-480 (Lane 3), treated with Nocodazole (500nM for 18 hrs) (Lane 4), COLO 205 (Lane 5), HCT 116 (Lane 6), K-562 (Lane 7), MDA-MB-231 (Lane 8), HepG2 (Lane 9), A549 (Lane 10) using GTX15779 PLK1 antibody [13E8].
Dilution : 1:1000

GTX15779 ICC/IF Image

ICC/IF analysis of U251 cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

GTX15779 WB Image

WB analysis of untreated or Nocodazole (100nM, 48 hours) treated U2OS lysate from non-targeting control or PLK1 siRNA transfected U2OS cells using GTX15779 PLK1 antibody [13E8].
Dilution : 1:1000
Loading : 25 μg

GTX15779 IHC-P Image

IHC-P analysis of human colon tissue using GTX15779 PLK1 antibody [13E8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8].
Progression of cells through subsequent phases of the cell cycle results in differential localization of PLK1 in spindle poles, kinetochores and cleavage furrow.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC

GTX15779 ICC/IF Image

ICC/IF analysis of WiDr cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty

PLK1 antibody [13E8]

Cat. No. GTX15779

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX15779 ICC/IF Image

GTX15779 ICC/IF Image

GTX15779 WB Image

GTX15779 WB Image

GTX15779 ICC/IF Image

GTX15779 WB Image

GTX15779 IHC-P Image

GTX15779 ICC/IF Image

GTX15779 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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