IP analysis of DDDDK tagged PLK1 overexpressed HEK293T lysate using GTX83865 PLK1 antibody [1D4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

IHC-P analysis of human pancreas carcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human bladder carcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human breast adenocarcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human kidney carcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human kidney tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human prostate carcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human lung tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

FACS analysis of HEK293T cells transfected with either PLK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83865 PLK1 antibody [1D4].
Dilution : 1:100

IHC-P analysis of human lung carcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human thyroid carcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human prostate tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human pancreas tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human endometrium tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human colon adenocarcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of HEK293T cells transfected with PLK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83865 PLK1 antibody [1D4].
Loading : 5 ug per lane
Dilution : 1:500

IHC-P analysis of human colon tissue using GTX83865 PLK1 antibody [1D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.