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PLK1 antibody [8C12]

Overexpression
Anti-PLK1 antibody [8C12] used in Western Blot (WB). GTX83866
Anti-PLK1 antibody [8C12] used in Flow cytometry (FACS). GTX83866
Anti-PLK1 antibody [8C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83866
Anti-PLK1 antibody [8C12] used in Immunoprecipitation (IP). GTX83866
Anti-PLK1 antibody [8C12] used in Western Blot (WB). GTX83866

Cat. No. GTX83866

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8C12

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all PLK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human PLK1 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 1 , PLK , STPK13

Cellular Localization

Nucleus

Background

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.

Database

Research Area

DATA IMAGES

Anti-PLK1 antibody [8C12] used in Western Blot (WB). GTX83866

GTX83866 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PLK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83866 PLK1 antibody [8C12].
Loading : 5 ug per lane
Dilution : 1:500

Anti-PLK1 antibody [8C12] used in Flow cytometry (FACS). GTX83866

GTX83866 FACS Image

FACS analysis of HEK293T cells transfected with either PLK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83866 PLK1 antibody [8C12].
Dilution : 1:100

Anti-PLK1 antibody [8C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83866

GTX83866 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PLK1 plasmid using GTX83866 PLK1 antibody [8C12].
Dilution : 1:100

Anti-PLK1 antibody [8C12] used in Immunoprecipitation (IP). GTX83866

GTX83866 IP Image

IP analysis of DDDDK tagged PLK1 overexpressed HEK293T lysate using GTX83866 PLK1 antibody [8C12].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-PLK1 antibody [8C12] used in Western Blot (WB). GTX83866

GTX83866 WB Image

WB analysis of various cell lines using GTX83866 PLK1 antibody [8C12].
Loading : 35 ug per lane
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519