APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-5μg/ml |
| 1:50-1:100 |
| 3-5μg/ 10⁶ cells |
| 5μg |
| 0.1-1μg/ml |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A His-tagged Plk fusion protein (C-term Plk1) corresponding to aa402-603 of the human Plk1 protein.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PLK , PLK1 , STPK13 , polo like kinase 1
Cellular Localization
Nucleus,Chromosome,Centromere,Kinetochore,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle,Midbody
Background
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210](/templates/default/js/lightbox/images/loading.gif "Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210")
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GTX14210 IHC-P Image
IHC-P analysis of human colon tissue using GTX14210 PLK1 antibody [Mixed clones].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX14210 IHC-P Image
IHC-P analysis of mouse colon tissue using GTX14210 PLK1 antibody [Mixed clones].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50
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GTX14210 WB Image
WB analysis of various samples using GTX14210 PLK1 antibody [Mixed clones].
Lane1 : HEK-293
Lane2 : K562
Lane3 : A549
Lane4 : HT-29
Lane5 : COLO 205
Lane6 : PC-12
Lane7 : Cos-7
Lane8 : NIH3T3
Lane9 : A-431
Lane10 : HeLa
Loading : 20μg
Dilution : 1-2μg/ml
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GTX14210 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX14210 PLK1 antibody [Mixed clones].
Dilution : 1μg/ml
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REFERENCE
There are currently no references for PLK1 antibody [Mixed clones] (GTX14210). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PLK1 antibody [Mixed clones] (GTX14210). Be the first to share your experience with this product.
![Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210](/upload/website/prouct_img/small/GTX14210/GTX14210_20191203_IHC-P_20_w_23060620_745.webp "Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210](/upload/website/prouct_img/small/GTX14210/GTX14210_20191203_IHC-P_21_w_23060620_531.webp "Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in Western Blot (WB). GTX14210](/upload/website/prouct_img/small/GTX14210/GTX14210_20191203_WB_41_w_23060620_283.webp "Anti-PLK1 antibody [Mixed clones] used in Western Blot (WB). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14210](/upload/website/prouct_img/small/GTX14210/GTX14210_20191203_ICC-IF_3_w_23060620_538.webp "Anti-PLK1 antibody [Mixed clones] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210](/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_IHC-P_20_w_23060620_745.webp "Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210](/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_IHC-P_21_w_23060620_531.webp "Anti-PLK1 antibody [Mixed clones] used in IHC (Paraffin sections) (IHC-P). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in Western Blot (WB). GTX14210](/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_WB_41_w_23060620_283.webp "Anti-PLK1 antibody [Mixed clones] used in Western Blot (WB). GTX14210")
![Anti-PLK1 antibody [Mixed clones] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14210](/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_ICC-IF_3_w_23060620_538.webp "Anti-PLK1 antibody [Mixed clones] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14210")