ICC/IF analysis of PFA-fixed Heat-shock treated HeLa cells using GTX03368 PLK1 (phospho Thr210) antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

IHC-P analysis of mouse colon tissue sections using GTX03368 PLK1 (phospho Thr210) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

WB analysis of extracts from HeLa cells treated with thymidine/nocodazole using GTX03368 PLK1 (phospho Thr210) antibody.
Lane 1 : primary antibody pre-treated with phospho-blocking peptide.
Lane 2 : primary antibody pre-treated with non-phospho-blocking peptide.

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03368 PLK1 (phospho Thr210) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml