PLK1 (phospho Thr210) antibody

Cat. No. GTX03368

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet

View product citations for antibody GTX03368 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
IHC-P	1:50-1:200
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from Human PLK1 (Accession P53350), corresponding to amino acid residues D194-V222.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 1 , PLK , STPK13

Cellular Localization

Nucleus

Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Database

Gene ID: 5347 PLK1
UniProt: P53350 PLK1

Research Area

DATA IMAGES

GTX03368 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03368 PLK1 (phospho Thr210) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

GTX03368 WB Image

WB analysis of extracts from HeLa cells treated with thymidine/nocodazole using GTX03368 PLK1 (phospho Thr210) antibody.
Lane 1 : primary antibody pre-treated with phospho-blocking peptide.
Lane 2 : primary antibody pre-treated with non-phospho-blocking peptide.

GTX03368 IHC-P Image

IHC-P analysis of mouse colon tissue sections using GTX03368 PLK1 (phospho Thr210) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03368 ICC/IF Image

ICC/IF analysis of PFA-fixed Heat-shock treated HeLa cells using GTX03368 PLK1 (phospho Thr210) antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

REFERENCE

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Package

List Price ($)

100 μl

$ 399

Qty