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PLK1 (phospho Tyr217) antibody

Orthogonal
Anti-PLK1 (phospho Tyr217) antibody used in Western Blot (WB). GTX18156

Cat. No. GTX18156

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all PLK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PLK1 around the phosphorylation site of Tyr217

Purification

Purified by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 1 , PLK , STPK13

Cellular Localization

Nucleus

Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PLK1 (phospho Tyr217) antibody used in Western Blot (WB). GTX18156

GTX18156 WB Image

Orthogonal

WB analysis of HeLa cell lysate preincubated with the phospho and nonphospho antigen peptide using GTX18156 PLK1 (phospho Tyr217) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399