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PLK2 antibody [C1C3]

Anti-PLK2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112022
Anti-PLK2 antibody [C1C3] used in Western Blot (WB). GTX112022

Cat. No. GTX112022

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PLK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 2 , SNK , hPlk2 , hSNK

Background

Serum-inducible kinase is a member of the 'polo' family of serine/threonine protein kinases that have a role in normal cell division.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PLK2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112022

GTX112022 IHC-P Image

PLK2 antibody [C1C3] detects PLK2 protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PLK2 antibody [C1C3] (GTX112022) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PLK2 antibody [C1C3] used in Western Blot (WB). GTX112022

GTX112022 WB Image

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX112022 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Guerber L et al. EMBO Rep 2023; 24 (6) : e56241 UBAP2L-dependent coupling of PLK1 localization and stability during mitosis.
Kim JH et al. Cell Signal 2013; 25 (4) : 805-13 Celastrol suppresses breast cancer MCF-7 cell viability via the AMP-activated protein kinase (AMPK)-induced p53-polo like kinase 2 (PLK-2) pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169