Untreated (–) and treated (+) U2OS whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody (GTX111754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PLK4(GTX111754) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody (GTX111754) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.