PLRG1 antibody [C3], C-term

Cat No. GTX106333

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($329), 25 μl ($169)

See all PLRG1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Raji

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241191

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pleiotropic regulator 1 , Cwc1 , PRL1 , PRP46 , PRPF46 , TANGO4

Cellular Localization

Nucleus speckle

Background

Necessary for spliceosome assembly and for pre-mRNA splicing.

Database

Gene ID: 5356 PLRG1
UniProt: O43660 PLRG1

DATA IMAGES

GTX106333 ICC/IF Image

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term (GTX106333) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106333 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106333 diluted at 1:1000

GTX106333 IHC-P Image

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PLRG1 antibody [C3], C-term (GTX106333) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 329

25 μl

$ 169

Qty