APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Raji
Predict Reactivity
Rat, Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MGC110980 antibody, PRL1 antibody, PRP46 antibody, PRPF46 antibody, PLRG1 antibody, "pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) antibody", pleiotropic regulator 1 antibody
Cellular Localization
Nucleus speckle
Background
Necessary for spliceosome assembly and for pre-mRNA splicing.
Database
DATA IMAGES
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GTX106333 ICC/IF Image
PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term (GTX106333) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX106333 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106333 diluted at 1:1000
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GTX106333 IHC-P Image
PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PLRG1 antibody [C3], C-term (GTX106333) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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