GeneTex
United States (US)

PLRG1 antibody [C3], C-term

GTX106333_39708_IFA_19011506_501.jpg
GTX106333_WB_19011506_241.jpg
GTX106333_39708_20141024_IHC_M_19011506_879.jpg

Cat No. GTX106333

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Raji

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC110980 antibody, PRL1 antibody, PRP46 antibody, PRPF46 antibody, PLRG1 antibody, "pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) antibody", pleiotropic regulator 1 antibody

Cellular Localization

Nucleus speckle

Background

Necessary for spliceosome assembly and for pre-mRNA splicing.

Database

DATA IMAGES
GTX106333_39708_IFA_19011506_501.jpg

GTX106333 ICC/IF Image

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term (GTX106333) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106333_WB_19011506_241.jpg

GTX106333 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106333 diluted at 1:1000

GTX106333_39708_20141024_IHC_M_19011506_879.jpg

GTX106333 IHC-P Image

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PLRG1 antibody [C3], C-term (GTX106333) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169