Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLZF antibody [N1N3] (GTX111046) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PLZF antibody [N1N3] detects PLZF protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLZF protein stained by PLZF antibody [N1N3] (GTX111046) diluted at 1:2000.
Blue: Hoechst 33342 staining.