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PLZF antibody [N1N3]

Anti-PLZF antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111046
Anti-PLZF antibody [N1N3] used in Western Blot (WB). GTX111046

Cat. No. GTX111046

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Chicken
Package
100 μl ($399),
25 μl ($169)
See all PLZF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLZF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169952

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger and BTB domain containing 16 , PLZF , ZNF145

Cellular Localization

Nucleus

Background

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PLZF antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111046

GTX111046 ICC/IF Image

PLZF antibody [N1N3] detects PLZF protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLZF protein stained by PLZF antibody [N1N3] (GTX111046) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-PLZF antibody [N1N3] used in Western Blot (WB). GTX111046

GTX111046 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLZF antibody [N1N3] (GTX111046) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30410445

REFERENCE

Application Reference

Yamanaka S et al. EMBO J 2021; 40 (4) : e105375 Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF.
Application : IHC-Fr
Reactivity : Chicken
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169