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PMCA ATPase antibody [5F10]

Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FACS). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FACS). GTX22825
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Western Blot (WB). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825

Cat No. GTX22825

Host Mouse
Clonality Monoclonal
Clone Name 5F10
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Neut
Reactivity Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Hamster, Chicken, Pig, Amphibians, Primate
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:100-1:200
IHC-P 1:500
IHC-Fr 1:500
FACS 1μg/test
IP Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

135 kDa. ( Note )

Specificity/Sensitivity

This antibody detects all four known isoforms of the PMCA ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified human erythrocyte calcium ATPase.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PMCA1,PMCA1kb

Cellular Localization

Cell membrane

Background

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
DATA IMAGES
Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FACS). GTX22825

GTX22825 FACS Image

FACS analysis of Hela cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FACS). GTX22825

GTX22825 FACS Image

FACS analysis of Jurkat cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825

GTX22825 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-PMCA ATPase antibody [5F10] used in Western Blot (WB). GTX22825

GTX22825 WB Image

WB analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3) lysates using GTX22825 PMCA ATPase antibody [5F10].
Dilution : 1:2000

Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825

GTX22825 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825

GTX22825 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825

GTX22825 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825

GTX22825 IHC-P Image

IHC-P analysis of human brain tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825

GTX22825 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

REFERENCE
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REVIEW
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Package List Price ($)
$ 329