PMCA ATPase antibody [5F10]

Cat. No. GTX22825
2
Cat. No.  GTX22825
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
GTX22825 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

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Anti-PMCA ATPase antibody [5F10] used in Western Blot (WB). GTX22825
GTX22825 WB Image

WB analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3) lysates using GTX22825 PMCA ATPase antibody [5F10].
Dilution : 1:2000

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Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
GTX22825 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

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Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
GTX22825 IHC-P Image

IHC-P analysis of human brain tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

4 / 9
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
GTX22825 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

5 / 9
Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FCM). GTX22825
GTX22825 FCM Image

FACS analysis of Jurkat cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

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Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
GTX22825 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

7 / 9
Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FCM). GTX22825
GTX22825 FCM Image

FACS analysis of Hela cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

8 / 9
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
GTX22825 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

9 / 9
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Western Blot (WB). GTX22825
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FCM). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22825
Anti-PMCA ATPase antibody [5F10] used in Flow cytometry (FCM). GTX22825
Anti-PMCA ATPase antibody [5F10] used in IHC (Paraffin sections) (IHC-P). GTX22825
This PMCA ATPase antibody [5F10] (GTX22825) is a mouse monoclonal antibody validated for detection of PMCA ATPase by WB, ICC/IF, IHC-P, IHC-Fr, FCM, IP, and Neutralizing /Inhibition. Reactivity has been shown in Human, Mouse, Rat, and other species.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    5F10
  • Isotype
    IgG2a
  • Applications
    WB ICC/IF IHC-P IHC-Fr FCM IP Neutralizing /Inhibition
  • Reactivity
    Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Hamster, Chicken, Pig, Amphibians, Primate
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