This antibody detects all four known isoforms of the PMCA ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified human erythrocyte calcium ATPase.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_384876
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
IHC-P analysis of human tonsil tissue using GTX22825 PMCA ATPase antibody [5F10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX22825 FACS Image
FACS analysis of Hela cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue). Dilution : 1 ug/test for 40 min at room temperature Fixation : 2% paraformaldehyde
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GTX22825 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 ºC
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GTX22825 FACS Image
FACS analysis of Jurkat cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue). Dilution : 1 ug/test for 40 min at room temperature Fixation : 2% paraformaldehyde
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GTX22825 ICC/IF Image
ICC/IF analysis of C6 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4ºC
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GTX22825 IHC-P Image
IHC-P analysis of human brain tissue using GTX22825 PMCA ATPase antibody [5F10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX22825 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX22825 PMCA ATPase antibody [5F10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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GTX22825 WB Image
WB analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3) lysates using GTX22825 PMCA ATPase antibody [5F10]. Dilution : 1:2000
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GTX22825 ICC/IF Image
ICC/IF analysis of U251 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4ºC