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PMCA1 antibody

Orthogonal
Anti-PMCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130858
Anti-PMCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130858
Anti-PMCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130858
Anti-PMCA1 antibody used in Western Blot (WB). GTX130858

Cat. No. GTX130858

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PMCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PMCA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886367

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase plasma membrane Ca2+ transporting 1 , PMCA1 , PMCA1kb

Cellular Localization

Cell membrane

Background

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PMCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130858

GTX130858 IHC-P Image

PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PMCA1 antibody (GTX130858) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PMCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130858

GTX130858 IHC-P Image

PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
PMCA1 antibody (GTX130858) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PMCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130858

GTX130858 IHC-P Image

PMCA1 antibody detects PMCA1 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: PMCA1 protein stained by PMCA1 antibody (GTX130858) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PMCA1 antibody used in Western Blot (WB). GTX130858

GTX130858 WB Image

Orthogonal

PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (GTX130858) at a dilution of 1:1000.

REFERENCE

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REVIEW

PMCA1 antibody Cat. No. GTX130858

Rating
( Average 4 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 2 users reviews)
Anti-PMCA1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130858
Date : Anonymous submitted on 26-Jul-2016
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : jejunum tissue
Blocking : 10% normal goat serum in PBST, 20°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:500, 4°C, 15Hr
Anti-PMCA1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130858
Date : Anonymous submitted on 26-Jul-2016
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : skeletal muscle tissue
Blocking : 10% normal goat serum in PBST, 20°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:500, 4°C, 15Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169