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PMCA2 ATPase antibody

Anti-PMCA2 ATPase antibody used in IHC (Paraffin sections) (IHC-P). GTX23529
Anti-PMCA2 ATPase antibody used in IHC (Paraffin sections) (IHC-P). GTX23529

Cat No. GTX23529

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

Human, Mouse, Rat, Chicken, Pig
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF Assay dependent
IHC-P 1:20-1:200
IHC-Fr 1:20-1:200
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

Product Note

This antibody detects an ~127 kDa protein and an ~133 kDa protein representing PMCA2a and PMCA2b ATPase, respectively, from rat brain microsomal fractions.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide: T(5) N S D F Y S K N Q R N E S S(19)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATPase plasma membrane Ca2+ transporting 2 , PMCA2 , PMCA2a , PMCA2i

Cellular Localization

Cell junction, synapse,Cell membrane

Background

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PMCA2 ATPase antibody used in IHC (Paraffin sections) (IHC-P). GTX23529

GTX23529 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX23529 PMCA2 ATPase antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-PMCA2 ATPase antibody used in IHC (Paraffin sections) (IHC-P). GTX23529

GTX23529 IHC-P Image

IHC-P analysis of human brain tissue using GTX23529 PMCA2 ATPase antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339