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PMS1 antibody

Anti-PMS1 antibody used in Immunoprecipitation (IP). GTX129232
Anti-PMS1 antibody used in Western Blot (WB). GTX129232

Cat No. GTX129232

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

293T,A431,HeLa,HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PMS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

PMS1 homolog 1, mismatch repair system component , HNPCC3 , MLH2 , PMSL1 , hPMS1

Cellular Localization

Nucleus

Background

This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype, which is also known as Lynch syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PMS1 antibody used in Immunoprecipitation (IP). GTX129232

GTX129232 IP Image

Immunoprecipitation of PMS1 protein from HeLa whole cell extracts using 5 μg of PMS1 antibody (GTX129232).
Western blot analysis was performed using PMS1 antibody (GTX129232) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PMS1 antibody used in Western Blot (WB). GTX129232

GTX129232 WB Image

PMS1 antibody detects PMS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with PMS1 antibody (GTX129232) diluted by 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169