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PMS2 antibody [N1N3]

Overexpression
Anti-PMS2 antibody [N1N3] used in Immunoprecipitation (IP). GTX113804
Anti-PMS2 antibody [N1N3] used in Western Blot (WB). GTX113804
Anti-PMS2 antibody [N1N3] used in Western Blot (WB). GTX113804

Cat. No. GTX113804

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PMS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

human PMS2-transfected 293T cells , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PMS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037723

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PMS1 homolog 2, mismatch repair system component , HNPCC4 , MLH4 , PMS2CL , PMSL2

Cellular Localization

Nucleus

Background

This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PMS2 antibody [N1N3] used in Immunoprecipitation (IP). GTX113804

GTX113804 IP Image

Immunoprecipitation of PMS2 protein from HeLa whole cell extracts using 5 μg of PMS2 antibody [N1N3] (GTX113804).
Western blot analysis was performed using PMS2 antibody [N1N3] (GTX113804) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PMS2 antibody [N1N3] used in Western Blot (WB). GTX113804

GTX113804 WB Image

PMS2 antibody [N1N3] detects PMS2 protein by western blot analysis.
A. 30 μg A431 whole cell extract
B. 30 μg HeLa whole cell extract
C. 30 μg HepG2 whole cell extract
7.5 % SDS-PAGE
PMS2 antibody [N1N3] (GTX113804) dilution: 1:1000

Anti-PMS2 antibody [N1N3] used in Western Blot (WB). GTX113804

GTX113804 WB Image

Overexpression

PMS2 antibody [N1N3] detects PMS2 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human PMS2-transfected 293T cells
7.5 % SDS-PAGE
PMS2 antibody [N1N3] (GTX113804) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169