Peptide:N-glycosidase F (PNGase F) removes all N-linked glycans (high-mannose, hybrid, and complex) from glycopeptides and glycoproteins. This PNGase F, a recombinant amidase cloned from Elizabethkingia miricola, cleaves between the innermost GlcNAc and asparagine residues. Protocols for both denaturing and non-denaturing conditions are shown in the "Application Note" section.

One vial of GTX141281-pro contains 75000 units (75kU) PNGase F.