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PNK antibody

Overexpression
Anti-PNK antibody used in Western Blot (WB). GTX24191
Anti-PNK antibody used in Western Blot (WB). GTX24191

Cat. No. GTX24191

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Chimpanzee
Package
100 μg ($589)
See all PNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1:500
ELISA 1:3000-1:12000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region of human PNK protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polynucleotide kinase 3''-phosphatase , AOA4 , EIEE10 , MCSZ , PNK

Cellular Localization

Nucleus

Background

Mammalian PNK catalyzes the 5-prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.

Database

Research Area

DATA IMAGES

Anti-PNK antibody used in Western Blot (WB). GTX24191

GTX24191 WB Image

Western blot using GeneTex's Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5).  Approximately 10 μg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature.  Signal was detected using standard techniques.

Anti-PNK antibody used in Western Blot (WB). GTX24191

GTX24191 WB Image

Overexpression

WB analysis of various samples using GTX24191 PNK antibody.
Lane 1 : Y190 yeast whole cell lysate
Lane 2 : PNK-overexpressed Y190 yeast whole cell lysate
Lane 3 : EM9 XH whole cell lysate
Lane 4 : PNK-overexpression EM9 XH whole cell lysate
Lane 5 : HeLa whole cell lysate
Loading : 10 μg
Dilution : 1:1000

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589