WB analysis of various samples using GTX24191 PNK antibody.
Lane 1 : Y190 yeast whole cell lysate
Lane 2 : PNK-overexpressed Y190 yeast whole cell lysate
Lane 3 : EM9 XH whole cell lysate
Lane 4 : PNK-overexpression EM9 XH whole cell lysate
Lane 5 : HeLa whole cell lysate
Loading : 10 μg
Dilution : 1:1000

Western blot using GeneTex's Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5).  Approximately 10 μg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature.  Signal was detected using standard techniques.