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PNPase antibody [4C11]

Anti-PNPase antibody [4C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70159
Anti-PNPase antibody [4C11] used in Western Blot (WB). GTX70159

Cat. No. GTX70159

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C11

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all PNPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human PNPase.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11177148

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polyribonucleotide nucleotidyltransferase 1 , COXPD13 , DFNB70 , OLD35 , PNPASE , old-35

Cellular Localization

Cytoplasm , Mitochondrion

Background

The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-PNPase antibody [4C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70159

GTX70159 ICC/IF Image

PNPase antibody [4C11] detects PNPase protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PNPase stained by PNPase antibody [4C11] (GTX70159) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-PNPase antibody [4C11] used in Western Blot (WB). GTX70159

GTX70159 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PNPase antibody [4C11] (GTX70159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399