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PNPase antibody

Anti-PNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX116542
Anti-PNPase antibody used in Western Blot (WB). GTX116542
Anti-PNPase antibody used in Western Blot (WB). GTX116542

Cat. No. GTX116542

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all PNPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Mouse liver , Jurkat

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PNPase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polyribonucleotide nucleotidyltransferase 1 , COXPD13 , DFNB70 , OLD35 , PNPASE , old-35

Cellular Localization

Mitochondrion

Background

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX116542

GTX116542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PNPase(GTX116542) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PNPase antibody used in Western Blot (WB). GTX116542

GTX116542 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX116542 diluted at 1:1000

Anti-PNPase antibody used in Western Blot (WB). GTX116542

GTX116542 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX116542 diluted at 1:1000

REFERENCE

Application Reference

Wang DD et al. J Biol Chem 2014; Helicase SUV3, Polynucleotide Phosphorylase PNPase, and Mitochondrial Polyadenylation Polymerase mtPAP Form a Transient Complex to Modulate Mitochondrial mRNA Polyadenylated Tail Lengths in Response to Energetic Changes.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169